ชุดทดลองการวิเคราะห์องค์ประกอบสเปกโตรโฟโตเมตรี (โฟโตเมตรีสีผสม)

Principle

In solutions containing different-coloured substances the concentrations of the dyes can be anlysed by spectrometry without prior separation of the substances.
Using the integrated software the spectra of the pure dye solutions and mixtures thereof will be recorded. Calibration curves for each substances enable us to determine the quantity of that substance in the solution.

Benefits

• Experimental procedure without dangerous chemicals
• Modern, easy-to-use photospectrometer
• Simple evaluation by supplied software

Tasks

In a mixed solution containing fuchsine acid and patent blue V, the proporion of fuchsine acid is to be determined.

Learning objectives

1. Photometry
2. UV-VIS spectrometry
3. Lambert-Beer’s law
4. Dyes
5. Absorption of light